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ID 15918

Transformation

Description:
DNA transformation is a naturally occuring but rare event in which DNA can be transferred into bacteria. In 1970, Morton Mandel and Akiko Higa discovered a way to make E. coli more "competent" for transforming foreign DNA. Their calcium chloride method is widely used today to obtain high-efficiency transforming cells.
Keywords:
dna transformation,calcium chloride,high efficiency,higa,rare event,akiko,e coli,mandel,bacteria,cells
Creative Commons License This work by Cold Spring Harbor Laboratory is licensed under a Creative Commons Attribution-Noncommercial-No Derivative Works 3.0 United States License.

Related content:

16705. Animation 34: Genes can be moved between species.
Stanley Cohen and Herbert Boyer transform bacteria with a recombinant plasmid, and Doug Hanahan studies induced transformation.
16715. Video 34: Douglas Hanahan, clip 1
Improving on the Mandel and Higa method of DNA transformation.
15916. DNA transformation
Stanley Cohen and Herbert Boyer inserted the recombinant DNA molecule they created into E. coli bacteria by means of a plasmid, thereby inducing the uptake and expression of a foreign DNA sequence known as "transformation."
16395. Animation18: Bacteria and viruses have DNA too.
Joshua Lederberg worked with bacterial genetics while Alfred Hershey showed that DNA is responsible for the reproduction of new viruses in a cell.
15162. Preparing the DNA for sequencing, Shane Yeager
Shane Yeager, from the Whitehead Institute Center for Genome Research, explains the processes of storing and preparing DNA for sequencing.
16687. Concept 33: Genes can be turned on and off.
Organisms can regulate gene expression.
16657. Animation 31: Some DNA does not encode protein.
Roy Britten presents his work with David Kohne on repetitive DNA and its evolutionary origins.
15638. Bacterium containing plasmids with foreign DNA
Illustration of bacterium containing plasmids with foreign DNA.
16720. Biography 34: Stan Norman Cohen (1935 - )
Stan Cohen and Herb Boyer "invented" recombinant DNA technology.
16613. Animation 28: Some types of mutations are automatically repaired.
Stan Rupert explains UV damage and light-activated DNA repair systems and Richard Setlow's work on thymine dimer repair.
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