Website Search
ID 15922
Early DNA sequencing
Description:
Two sequencing techniques were developed independently in the 1970s. The method developed by Fred Sanger used chemically altered "dideoxy" bases to terminate newly synthesized DNA fragments at specific bases (either A, C, T, or G). These fragments are then size-separated, and the DNA sequence can be read.
Keywords:
fred sanger,dna fragments,dna sequencing,dna sequence,1970s
This work by Cold Spring Harbor Laboratory is licensed under a Creative Commons Attribution-Noncommercial-No Derivative Works 3.0 United States License.Related content:
- 15923. Cycle sequencing
- The sequencing method developed by Fred Sanger forms the basis of automated "cycle" sequencing reactions today. Fluorescent dyes are added to the reactions, and a laser within an automated DNA sequencing machine is used to analyze the DNA fragments produc
- SOURCE: DNAi
- 16036. Fred Sanger, 1975
- A gene is a discrete sequence of DNA nucleotides
- SOURCE: DNAi
- 15479. Sanger method of DNA sequencing, 3D animation with narration
- The DNA sequencing method developed by Fred Sanger forms the basis of automated "cycle" sequencing reactions today.
- SOURCE: DNAi
- 15975. Human and mtDNA variation
- When comparing Neandertal and human mtDNA, researchers found that the differences between the sequences put Neandertals outside the range of variation of modern humans.
- SOURCE: DNAi
- 15976. Neandertal and human (mtDNA)
- When comparing Neandertal and human mtDNA, researchers found that the differences between the sequences put Neandertals outside the range of variation of modern humans.
- SOURCE: DNAi
- 15912. Sequencing DNA
- Techniques to read the sequence of DNA, letter by letter, have been available since the 1970s. However, the massive task of sequencing the three billion basepairs of the human genome required machines that could read and interpret the data.
- SOURCE: DNAi
- 16515. Animation 23: A gene is a discrete sequence of DNA nucleotides.
- Fred Sanger outlines DNA sequencing.
- SOURCE: DNAFTB
- 15921. Gel electrophoresis
- In the early days of DNA manipulation, DNA fragments were laboriously separated by gravity. In the 1970s, the powerful tool of DNA gel electrophoresis was developed.
- SOURCE: DNAi
- 15567. DNA sequence
- Early sequencers used four different reactions to determine the placement of each of DNA's four bases - known as A, C, T & G - in the sequence.
- SOURCE: DNAi
- 15573. DNA sequencer
- An image of a DNA sequencer.
- SOURCE: DNAi
