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How Embryonic Stem Cell Lines are Made

Use of embryonic stem cells in research has been hotly debated for several years. This animation presents the basics on how stem cell lines are established. For more information on how techniques similar to this are used in research.
Embryonic stem cells are derived from blastocysts — embryos that are about a week old. At this stage, the blastocyst has about 100 cells. Human blastocysts like this have been donated to research from in vitro fertilization clinics. In order to get embryonic stem cell lines, scientists remove cells from the inner cell mass region. These cells have the potential to develop into any type of cell in the body. Once the cells are removed, they are placed on a culture plate with nutrients and growth factors. The blastocyst is destroyed in this process. An embryonic cell line is established when these cells multiply and divide. Under the right conditions, these cell lines can be maintained indefinitely. By adding different growth factors, it is possible to induce these embryonic stem cells into developing into different cell types. These cells could someday be used in therapies to replace damaged cells and organs.
stem cell, blastocyst, ethics
Creative Commons License This work by Cold Spring Harbor Laboratory is licensed under a Creative Commons Attribution-Noncommercial-No Derivative Works 3.0 United States License.

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